Continents

3D Reconstruction of Skin and Spatial Mapping of Immune Cell Density, Vascular Distance and Effects of Sun Exposure and Aging via Communications Biology

Mapping the human body at single cell resolution in three dimensions (3D) is important for understanding cellular interactions in context of tissue and organ organization. 2D spatial cell analysis in a single tissue section may be limited by cell numbers and histology. Here we show a workflow for 3D reconstruction of multiplexed sequential tissue sections: MATRICS-A (Multiplexed Image Three-D Reconstruction and Integrated Cell Spatial – Analysis). We demonstrate MATRICS-A in 26 serial sections of fixed skin (stained with 18 biomarkers) from 12 donors aged between 32–72 years. Comparing the 3D reconstructed cellular data with the 2D data, we show significantly shorter distances between immune cells and vascular endothelial cells (56 µm in 3D vs 108 µm in 2D). We also show 10–70% more T cells (total) within 30 µm of a neighboring T helper cell in 3D vs 2D. Distances of p53, DDB2 and Ki67 positive cells to the skin surface were consistent across all ages/sun exposure and largely localized to the lower stratum basale layer of the epidermis. MATRICS-A provides a framework for analysis of 3D spatial cell relationships in healthy and aging organs and could be further extended to diseased organs.

 

Read more…

Recent Posts

VivaTech 2025: Givaudan spotlights digital story-smelling and tech-driven fragrances via Premium Beauty NewsVivaTech 2025: Givaudan spotlights digital story-smelling and tech-driven fragrances via Premium Beauty News

VivaTech 2025: Givaudan spotlights digital story-smelling and tech-driven fragrances via Premium Beauty News

Beatrice Wihlander 11 June 2025 Givaudan is showcasing its fragrance technologies, which are underscored by…

ISO 23675: A new validated standard for SPF determination by Zurko ResearchISO 23675: A new validated standard for SPF determination by Zurko Research

ISO 23675: A new validated standard for SPF determination by Zurko Research

After over 10 years of testing sunscreen products, at Zurko Research we are proud to…

This sticker reads emotions (even the ones you try to hide) via Popular ScienceThis sticker reads emotions (even the ones you try to hide) via Popular Science

This sticker reads emotions (even the ones you try to hide) via Popular Science

Mack Degueurin 22 April 2025 The wearable device analyzes the tiny changes in physical responses…