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Co-culture of iNeurons with primary human skin cells provides a reliable model to examine intercellular communication via Wiley

Neurons communication with skin cells confer to the tissue the ability to undergo adaptive modifications during response to environmental changes or wound healing after injury. Thought for a long time to be dedicated to the central nervous system, the glutamatergic neuromodulation is increasingly described in peripheral tissues.

In this study, we performed small molecule-driven direct conversion of human skin primary fibroblasts into induced neurons (iNeurons).

Here, we report that iNeurons and primary skin cells established contacts, with neurite ensheathment by keratinocytes, and demonstrated that iNeurons cocultured with primary skin cells provide a reliable model to examine intercellular communication.

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