How to Claim on the Impact of a Cosmetic Product at the Functional Level of Skin and its Microbiota by Phylogene

10 October 2018

Recent metagenomic studies have demonstrated that the overall functional potential of the microbiome is rather conserved between healthy individuals.

A new study(1), in the intestinal tract, another microbiota, assess the biological processes undertaken in-vivo by microbes and the host by conducting a metaproteome analysis, as proteomics can reach the really impacted functions.

By analysing the proteins from samples of 16 healthy individuals at three measurement time points, half receiving a probiotic and half a placebo, the obtained metaproteome was found to contain a functional core but also to show variation across individuals and with time. The study could also show the human-bacterial interactions and the activity of specific microbial groups, by crossing 16S rDNA and metaproteomics informations.

It indicates that with a stable proportion of main functionalities, there is a “healthy balance”, e.g. in protein turnover and replication, in the intestinal ecosystem.

This kind of approach is the way to understand what are at molecular level the impact of a cosmetic or a probiotic on skin and microbiome functions together, and to claim on the healthy balance of skin and its microbiota functions.

(1)Faecal Metaproteomic Analysis Reveals a Personalized and Stable Functional Microbiome and Limited Effects of a Probiotic Intervention in Adults.

Kolmeder CA and all. PLoS One. 2016 Apr 12;11(4) https://www.ncbi.nlm.nih.gov/pubmed/27070903

With MS Phylogene, you can characterize and understand the effects on host and microbiome:

Bacterial 16s rDNA and fungal ITS sequencing with OTU-based estimation to investigate microbial communities diversity and determine taxonomic composition,

coupled with High-resolution nano LC-MS/MS quantitative proteomics and CORAVALID™/ MicroXplore^TM data processing.