Skin proteomic by LC-MS/MS: Now a classic workflow? by PHYLOGENE

4 September 2015

A new study (1) on psoriasis used the same way of untargeted relative quantitative comparison of skin proteomes (psoriasis patient vs control) by LC-MS/MS to discover thousands of proteins.

Among these, tens were moving and were followed by targeted LC-MS/MS SRM method. Many such studies were published during the 4 last years (2).

It seems now this is a good way to understand how an ingredient is active, and the understanding of actions on metabolic pathways allows to generate efficient supporting data for claims.

Quantitative tandem mass-spectrometry of skin tissue reveals putative psoriatic arthritis biomarkers. Cretu D. and all. 2015 http://www.ncbi.nlm.nih.gov/pubmed/25678896
See for example Skinobs 26/05/2015. UVB biomarkers : Another new study showed the interest of quantitative L-MS/MS, the study of Moon E and all. 2015 – http://www.ncbi.nlm.nih.gov/pubmed/25576851.

This way, you can also easily check the effects of your active ingredients on skin or cells with MS Phylogene support:

– High-resolution nano LC-MS/MS quantitative proteomics and CORAVALID™ data processing: The efficient discovery tool

– High-resolution SRM LC-MS/MS quantitative proteomics: The efficient multiplex confirmatory method