Various factors can alter human skin microbiome, a state referred to as dysbiosis.
The cosmetics should respect and maintain the microbiota. Thanks to DNA sequencing techniques is possible to analyse the skin bacterial community.
The study skin area is swabbed with sterile swabs. The genomic DNA from the swab heads is extracted using Ion 16S Metagenomics Kit (ThermoFisher Scientific).
The kit uses two primer pools to amplify seven hypervariable regions (V2, V3, V4, V6, V7, V8, and V9) of bacterial 16S rRNA. With this innovative test is possible to study the changes in the skin microbiome after testing cosmetic products.
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