M. Tarnowska, S. Briançon, J. Resende de Azevedo, Y. Chevalier, C. Barratier, T. Pourcher, M.‐A. Bolzinger
In vitro assessments of skin absorption of xenobiotics are essential for toxicological evaluations and bioavailability studies of cosmetic and pharmaceutical ingredients. Since skin metabolism can greatly contribute to xenobiotic absorption, experiments need to be performed with skin explants kept viable in suitable survival media. Existing protocols for non‐viable skin are modified to consider those conditions.
The objective was to design a survival medium used as an acceptor fluid in Franz cells for testing cutaneous penetration of hydrophilic or lipophilic molecules. Their metabolism inside skin may be investigated under the same conditions.
The determining factors involved in survival mechanisms in vitro are discussed. The consequences of short‐term skin preservation at 4°C were also evaluated.
The metabolic activity of fresh skin samples mounted in Franz cells was studied by measurement of lactate release over 24 h in order to assess the impacts of pH, buffering, osmolality, ionic strength, initial glucose supply and the addition of ethanol or non‐ionic surfactant in the acceptor part of Franz cells.
Parts of this work were presented during Skin Forum Annual Meeting, 20‐21 June 2018, Tallin, Estonia
Published by Online Library
Organized by the Société Française de Cosmétologie and supported by major names in the international…
Shruti Ghosh 3 April 2025 American cosmetic trends are changing fast. Imagine you’re getting ready…
19 May 2025 The beauty industry is undergoing significant transformation, driven by sustainability, innovation, and…