AOP has developed innovative live cell efficacy tests for assessment of intracellular antioxidant activity of active ingredients, extracts, or finished products. Using a combination of cell-based assays, the cellular mechanism of action and complete antioxidant profile of products can be investigated.

Direct antioxidant effect such as intracellular ROS/free radical scavenging, membrane scavenging activity, catalase-like effect and indirect antioxidant effect such as activation of natural protective cell antioxidant system ARE Nrf2 pathway are assessed.

AOP assays are available on different cell models. AOP provide quantitative data (dose-response curves, EC50, EC90, EC10) and comparison to standards to allow scientific claim support independently or before clinical trials.

AOP1 fluorescence kinetic profiles (top) obtained for each quercetin concentration (500-1.95 μM) and dose-response curve obtained after sigmoid fit (bottom).

EC₅₀ was evaluated at 17.20 µM with an R² = 0.998.

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CONTACT

Cécile Dufour

PhD

cdufour@antioxidant-power.com

www.antioxidant-power.com