With the CellSpectrometer CSM 2100, you have a compact and powerful impedance spectrometer with 12 or 24 measurement channels at your disposal, allowing you to assess the full impedance spectrum or the TEER (Transepithelial/transendothelial Electrical Resistance() of tissue barriers of a corresponding number of cell cultures simultaneously with exceptional ease and speed for many different application fields.

The CellSpectrometer CSM 2100 measures the resistance of a cell tissue layer to an alternating current over a range of frequencies (from 1 Hz to 200 KHz by 48 discrete frequency, logarithmically distributed across the entire spectrum). By determining how much electrical signal is blocked by the cellular layer, the measurement effectively assesses barrier integrity.

Typically, an impedance/TEER measurement setup involves a well plate with multiple inserts containing cultured tissue, wetted on both sides with an electrolyte medium. These inserts isolate the electrolyte medium surrounding the cell culture from the outer electrolyte medium, connecting the two only through the cell layer (see drawing). During measurement, an alternating current passes through two electrodes placed inside and outside the inserts. Real-time measurements of voltage, current, and phase shift (φ) are used to calculate the electrical resistance (in Ohms, Ω).

This kind of evaluation is standard in many bio-/medical applications for e.g. permeability, absorption, drug delivery, toxicology, maturity examination of cell cultures, cancer research, assessing the impact of nanoparticles in the environment on the human body, food safety and many more.
Due to the high sensitivity of impedance spectrometry to micro-damages in cell layers, TEER is used as an ethically acceptable and reliable standard for classifying potentially dangerous or toxic chemical substances: In accordance with guidelines like REACH (Registration, Evaluation, Authorization and Restriction of Chemicals) or the OECD Guidelines for the Testing of Chemicals, TEER measurement replaces animal tests such as the notorious Draize eye test performed on rabbits¹.

Advantages of the CellSpectrometer CSM 2100:

• – Transepithelial/transendothelial Electrical Resistance (TEER) measured at 12.5 Hz and 1000 Hz available in only 15 seconds.
• – Full spectrum: measurements across the entire spectrum from 1 Hz to 200 KHz for all cell cultures in just 100 seconds.
• Preventing cross-contamination of wells
• – Compatible with many standard 12 or 24 well plates and standing or hanging inserts.
• – Examples of suitable cell cultures include, all kind of ephithelial/endothelial tissue,  RhCE (Reconstructed Human Corneo-like Epithelium), epithelial/endothelial cells, skin models, other tissue-specific cells
• – Unique patented Titanium Nitride coated electrodes offer a significant improvement in signal quality. The electrodes cause up 50 x less resistance & phase shift compared to conventional electrodes made out of gold or stainless steel.
• – Self-contained system: The CellSpectrometer CSM 2100 operates independently without the need for a specialized environment, making it convenient for use in various laboratory settings (e.g. in an incubator).

Beside the easy to use device, the user-friendly software with many helpful features simplify your experiments. Every step in your workflow, from setting up your experiment to analyzing the generated data is fully supported by the application.

1: N. Knetzger, N. Ertych, T. Burgdorf, J. Beranek, M. Oelgeschläger, J. Wächter, A. Horchler, S. Gier, M. Windbergs, S. Fayyaz, F.A. Grimm, G. Wiora, C. Lotz, Non‑invasive in vitro NAM for the detection of reversible and irreversible eye damage after chemical exposure for GHS classification purposes (ImAi), Archives of Toxicology, January 2025

CONTACT

Diana Khazaka, General Manager

Georg Khazaka, General Manager

+49-221-9564990

info@courage-khazaka.de

https://www.courage-khazaka.com/en/