Intracellular and extracellular oxidative stress initiated by reactive oxygen species (ROS) advance skin aging, which is characterized by wrinkles and pigmentation. The use of the cosmetics containing antioxidants is known to help alleviate skin aging.
Ellead have been conducting the test using various method of in-vitro and in vivo. There are in vitro test methods for not only ingredients – ABTS assay, Total polyphenol content, SOD assay, DPPH assay – but also finished products through ORAC assay.
Another antioxidant evaluation worth noting is Carbonylated protein detection assay, that is non-invasive method for measuring carbonylated protein caused by the product of oxidative stress found in the stratum corneum of the skin. The detection method allows visualization of the stratum corneum carbonylated. (Fig 1.)
Fig 1. The florescence images of carbonylated protein detection of the stratum corneum. a) baseline, b) after 4 weeks of the cosmetics application
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